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Volume 64, Number Supplement, 2012
Light and Transmission Electron Microscopic Study of Spinal Cord in Embalmed and Fresh Cadavers
Yadaridee Viravud, D.D.S., M.Sc.,Vasana Palakornkul, D.V.M., M.Sc.,Kesorn Sripaoraya, M.Sc.,Kriengkrai Urusopon, M.D.
Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
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Abstract

Objective : To observe and differentiate histological structures and ultrastructures by light microscope and transmission electron  microscope  between embalmed cadaveric spinal cord  and fresh cadaveric spinal cord.               
Methods :  The spinal cords were dissected  from seven embalmed cadavers of the Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University. The specimens were prepared for routine hematoxylin and eosin (H&E) staining for light microscope (LM)study. Some of the specimens were prepared to study by transmission electron microscope (TEM). Finally we observe and compare between spinal cord from embalmed cadaver and fresh cadaver. Results :   By viewing with light microscope, the quality of spinal cords from embalmed cadavers are better and clearer than  from fresh cadavers.  Viewed  by  transmission  electron microscope, the lipid part in the spinal cord from  cadaveric embalmed tissues is degenerated  but  the  protein  part is not deformed. Conclusion : The spinal  cords  from cadavers which  were fixed by excess formalin injection and embalmed in  formalin  for 1-2 years , are well-preserved  for hematoxylin and eosin (H&E) staining techniques for light microscope (LM) study in  Histology.  However in the EM of spinal cord sections from embalmed cadavers, the protein component was more likely withstand to formalin and embalmment procedures compared to the lipid componennt (e.g.myelin sheath, cell membrane).

Keywords: Spinal cord, embalmed cadaver, fresh cadaver ,LM,TEM
Page: 61 - 64



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