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Volume 51, Number 10, 1999
Comparative Analysis of Venous Blood Lactate Levels by Automatic Lactate Strip Kit (Accusport) and by Standard Marbach?s Enzymatic Method
Kanokkrin Boonvisudhi, M.D.,Sarawut Junnu, B.Sc.,Rungtip Soi-ampornkul, Ph.D.,Rungnapa Supavilai, M.Sc.,Uchanee Wongmanee, B.Sc.,Narumol Panichkul, Dip,Neelobol Neungton, M.D.
*Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
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Abstract
Lactic acidosis is an emerging life-threatening condition that needs to be diagnosed and treated as early as possible. The complete analysis of blood lactate levels by standard method takes at least a few hours and is not available at all times. The automatic lactate strip kit (Accusport) will be more practical for diagnosis and treatment of lactic acidosis patients. This study showed the results of blood lactate determined by standard enzymatic method of Marbach compared to the lactate strip. The results showed a strong correlation between the two methods (r2=0.966). The correlation increased in the case of high blood lactate levels (r2=0.978) and decreased in normal blood lactate levels (r2=0.943). Overall lactate values measured from the strip method were lower than those from the enzymatic method. From this study we can calculate a constant factor of 0.981 which when multiplied with the value of blood lactate analysed by lactate strip then added with 0.532, the result will be equal to that from Marbach’s enzymatic method.
Keywords: Comparative analysis; Venous blood lactate levels; Automatic lactate strip kit; Standard Marbach?s enzymatic method
Page: 791 - 799



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